Signalling

Part:BBa_K511825:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible Notch-Gal4-ESN Receptor Generator (UAS-Gal4-Notch-Gal4-ESN) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
    Illegal NotI site found at 5458
    Illegal NotI site found at 5466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal BamHI site found at 3167
    Illegal XhoI site found at 83
    Illegal XhoI site found at 5749
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 492
    Illegal PstI site found at 897
    Illegal PstI site found at 1227
    Illegal PstI site found at 1458
    Illegal PstI site found at 1555
    Illegal PstI site found at 2745
    Illegal PstI site found at 4623
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 2870
    Illegal NgoMIV site found at 2926
    Illegal NgoMIV site found at 2969
    Illegal NgoMIV site found at 3754
    Illegal NgoMIV site found at 4771
    Illegal NgoMIV site found at 4948
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5668
    Illegal BsaI.rc site found at 327
    Illegal BsaI.rc site found at 2035
    Illegal BsaI.rc site found at 2950
    Illegal BsaI.rc site found at 5407


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References